Kevin W. McEnery, M.D.
Lucky K. Kelley, B.A.
Elizabeth A. Kelly, MLS
S. Bruce Dowton, M.D. (Syd.)
The raw visible human data set consists of over 1878 images 4 Mb compressed images and uncompressed 7 Mb of data per image. For the medical educator, while these images are of high detail, the 7 Mb images make them impractical to efficiently browse and manipulate.
As supplement medical education, we determined that every 5th image (5mm increment) was sufficient for illustration of cross-sectional anatomy. A total of 375 images were retrieved via the Internet from the National Library of Medicine FTP site. Uncompressed this data set would have required 2.6 Gb of image data storage. However, we wanted to maintain the entire project on an available computer with a 1 Mb hard drive.
We chose to reduce total image storage requirements through a combination of cropping, reducing the background to a single color, and reducing the color depth to 16 bit and 8 bits for Internet distribution. Other types of compression are available including JPEG and GIF. GIF compression was ultimately used for distribution because it could be viewed directly with any available WWW browser.
The raw image size of an individual image was 2048 x 1206. It was determined that cropping the raw images, a standard image size of 1771 x 1021 at 16 bits/pixel. We chose this size as the smallest size which would completely accommodate the largest thorax image with both arms included.
To allow viewing of the images in the same orientation as is standard for medical imaging, the images were flipped in both the horizontal and vertical plane.
An important aspect of the color reduction process was the elimination of the various blue colored hues of the surrounding blue ice enclosure and replacement with a solid black background. This was essential in the final reduction to 8 bit color. As the background was represented by a single color (black), the 256 color look-up table could represent the natural brown hues of the cross sectional images. Without this color reduction, the color table had large predominance of the blue hues found in the surrounding ice resulting in visible deterioration of image quality.
A Power Macintosh 7200 with Ethernet connection accomplished the downloading and subsequent image processing. Fetch was the FTP application. The same Macintosh computer functions as the image server for the project.
This entire process of downloading, decompression, image cropping, background color reduction, color table reduction/optimization, flipping the images from top to bottom and left to right, was achieved entirely through the scripting of these various program applications. These included Fetch (for FTP), PhotoShop, and Debabelizer (image processing). The entire 374 image data set was downloaded over the course of a three day week-end without an operator in attendance. The final full-resolution, 16 bit image set is approximately 390 Mb. Several anatomy focused image sets were abstracted from this reduced size master image set. (Table 1).
In interacting with the program, the user selects the anatomic area and then the exact level to be viewed by clicking on the drawing icon at the level to be viewed. The prior and next image in the series can be selected. If a user wanted a full resolution image, a single click on the anatomic image would retrieve the full resolution image. This could be saved by the user for incorporation in to other projects.
The network bandwidth saved by this approach is substantial. The user can view the image series and then determine the exact areas to be studied. The navigation icon images are about 70 Kb each. It is assumed that while there would be extensive interaction with the icon images, only those high resolution images that are of specific interest to the user will be downloaded.
| Number | Pixel Size | Image Size | ||
| Entire image set | Single | Total< /B> | ||
| Compressed | 1878 | 2048 x 1206 | 4 Mb | 7512 Mb |
| Non-compressed | 1878 | 2048 x 1206 | 7 Mb | 13176 Mb |
| Selected images | ||||
| Compressed | 375 | 2048x1206 | 4 Mb | 1500 Mb |
| Non-compressed 24bit | 375 | 2048 x 1206 | 7 Mb | 2625 Mb |
| Cropped 16 bit color | 375 | 1021 x 771 | 96 Kb - 2 Mb | 390 Mb |
| Number | Pixel size | Image size Kb (8 bit GIF) | ||
| Index images | 390 x 260 | 55-100 | ||
| Head & Neck | 75 | 649 x 729 | 100-357 | |
| Thorax | 46 | 990 x 838 | 638-867 | |
| Abdomen | 101 | 1090 x 1021 | 800-950 | |
| Spine | 151 | 470 x 455 | 179-255 | |
| Lower Extremity | 210 | 660x833 - 300x350 | 383-459 |
Illustration 1. Interface appearance of the navigation, interface. Image size of 60 Kb makes navigation through the images practical even with a modem. Clicking on the image downloads a "full resolution image". Left icon indicates approximeate level of current image.