Author: Alexa M. Salsbury, PhD

iCn3D Shortcuts and Help Docs

 

1. Go to iCn3D: Web-based 3D Structure Viewer

2. Input your PDB and AlphaFold IDs, "3NOS,P29474", and Click on Load Biological Unit

3. Orient yourself to the structures

Change style, color, and orientation to your liking

4. Realign Structure

 

5. Select 3NOS_A +Ctrl P29474_A

 

6. Select 3NOS_A + Alt + P29474_A and click on Realign with TM-align

 

7. View alignment

 

8. Change Color to "AlphaFold Confidence"

 

9. View full structure by turning off "Selection"

 

10. Click on View Selection for best view of the alignment

 

11. View Structure and color-coordinated sequence

 

 

 

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